ELISA Pro Human TNF-α

CYTOKINE ELISA KIT

ELISA Pro: Human TNF-α

ELISA kit designed for the quantitative measurement of human tumor necrosis factor alpha (TNF-α) in serum, plasma, and cell culture supernatants. Suitable for cytokine analysis in immunology and inflammation research.

CAT: 0930-3512-1HP-1 1 Plate Research Use Only
ELISA Pro Human TNF-alpha ELISA kit, 1 plate, catalog 0930-3512-1HP-1

Catalog reference: 0930-3512-1HP-1

Product Overview

ELISA Pro: Human TNF-α is an enzyme-linked immunosorbent assay (ELISA) kit intended for the quantitative measurement of human tumor necrosis factor alpha in serum, plasma, and cell culture supernatants.

TNF-α is a pro-inflammatory cytokine involved in immune signaling, inflammatory responses, and the regulation of various cellular processes. Measuring TNF-α helps researchers investigate cytokine production and inflammatory pathways in experimental systems.

For serum and plasma samples, the kit includes an ELISA diluent designed to block heterophilic antibodies from cross-linking assay antibodies, helping reduce interference that may contribute to false-positive readouts.

Intended use

Quantification of human TNF-α in serum, plasma, and cell culture supernatants. For research use only.

KIT SIZE 1 Plate
TARGET Human TNF-α
DRY ICE Not required
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Shelf life: at least 12 months from the date of receipt, according to the supplied product information.

Key Features & Benefits

Human TNF-α Quantification

Designed for quantitative measurement of human tumor necrosis factor alpha in compatible samples.

Multiple Sample Types

Intended for serum, plasma, and cell culture supernatants.

Interference Control

Includes an ELISA diluent formulated to reduce heterophilic antibody interference.

Inflammation Research

Supports studies investigating cytokine signaling and inflammatory responses.

About Human Tumor Necrosis Factor Alpha (TNF-α)

Tumor necrosis factor alpha is a cytokine involved in inflammatory signaling and immune system regulation. It participates in cellular communication and can influence immune cell activation and other biological responses.

TNF-α quantification is commonly used in research investigating inflammation, immune responses, cytokine secretion, and cellular reactions to experimental treatments. Measuring TNF-α levels can help researchers compare experimental groups and characterize changes in inflammatory signaling.

Supported Sample Types

Serum

For measuring human TNF-α in serum samples prepared according to the kit protocol.

Plasma

For quantifying TNF-α in plasma samples using the recommended sample preparation procedure.

Cell Culture Supernatants

For investigating TNF-α production in experimental cell culture systems.

ELISA Diluent and Heterophilic Antibody Interference

Heterophilic antibodies can interact with assay antibodies and interfere with immunoassay measurements, potentially producing false-positive signals. These antibodies are commonly found in human serum and plasma and may also occur in samples from other species.

According to the supplied product description, the ELISA diluent included in this kit blocks heterophilic antibodies from cross-linking assay antibodies. The diluent has been validated using serum and plasma samples from healthy human blood donors.

The diluent is designed to help reduce interference; it should not be assumed to eliminate every possible source of nonspecific signal. Follow the manufacturer's protocol and use appropriate experimental controls.

Technical Specifications

Product Name ELISA Pro: Human TNF-α
Catalog Number 0930-3512-1HP-1
Pack Size 1 Plate
Target Analyte Human tumor necrosis factor alpha (TNF-α)
Assay Type ELISA
Intended Sample Types Serum, plasma, cell culture supernatants
Included Diluent ELISA diluent designed to reduce heterophilic antibody interference
CAS Number Supplied 7732-18-5
Shelf Life At least 12 months from date of receipt
Dry Ice Required No
Hazardous No, according to the supplied product listing. Consult the applicable safety documentation.

Data note: CAS 7732-18-5 corresponds to water, not TNF-α. Verify its intended association with the kit against the manufacturer's documentation.

Research Use Only

This ELISA Pro kit is intended for research use only and is not for use in diagnostic procedures. Follow the manufacturer's instructions for sample preparation, assay execution, storage, and interpretation of results.

Explore Human TNF-α ELISA Testing

Request pricing for ELISA Pro: Human TNF-α, catalog 0930-3512-1HP-1, supplied as a one-plate kit for cytokine research.

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Stock items: 24/48H | Non-stock items: 2–6 weeks

2nd Oct 2026

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