CK2; Casein Kinase 2
Source: Bovine kidney.
Purity: > 90% by SDS-PAGE, subunit apparent Mr ~ 39- and 26-kDa.
Supplied: 1 μg in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 50% glycerol.
Activity: ~ 2400 units/mg with casein as substrate. One unit is the amount of the CK2 that incorporates 1 nmol of phosphate into casein per min. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Background: Casein Kinase 2 (CK2) is a tetrameric protein serine/threonine kinase that is ubiquitously distributed in eukaryotic cells and occurs in the cytoplasm, nucleus and mitochondria. The enzyme is an important component of signal transduction pathways, regulates cell growth, division and differentiation, and the activities of a variety of oncoproteins and transcription factors including c-Myc, c-Myb, serum response factor, c-Fos and c-Jun (GLO127-001, GLO127-005, GLO127-010). The kinase also plays an important role in cell survival and protects regulatory proteins from caspase-mediated degradation in apoptosis. Individual CK2 subunits have been recently shown to exist outside tetrameric complexes. Additionally, CK2 was reported to display dual specificity protein kinase activity.