7-Propargylamino-7-deaza-dATP-Cy5

Fluorescently Labeled Nucleotide for DNA Synthesis and Molecular Biology Applications

Product Description

7-Propargylamino-7-deaza-dATP-Cy5 is a fluorescently labeled deoxyadenosine triphosphate designed for incorporation into DNA during enzymatic synthesis. The attached Cy5 fluorescent dye enables the detection and analysis of labeled DNA products in molecular biology and fluorescence-based applications.

This modified nucleotide is suitable for incorporation through primer extension and PCR, supporting fluorescent DNA labeling and downstream analytical workflows.

General Laboratory Use: For general laboratory use. Please centrifuge briefly before opening (volume ≤ 2 mL).

Key Features

• Cy5 fluorescently labeled nucleotide
• Designed for enzymatic DNA incorporation
• Suitable for primer extension and PCR
• High purity of ≥ 95% (HPLC)
• Supplied as a solution in 10 mM Tris-HCl
• Concentration of 1.0–1.1 mM
• Blue-colored fluorescent nucleotide solution

Applications

• Incorporation by primer extension [1]
• Incorporation by PCR [2]
• Fluorescent DNA labeling
• Fluorescence-based DNA detection and analysis
• Molecular biology research

Technical Specifications

Spectroscopic Properties

Cy5 fluorescence properties support the detection and analysis of fluorescently labeled DNA in suitable optical systems.

Chemical Structure Information

SMILES:

[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c2c(c(c1)C#CCNC(=O)CCCCCN1c3ccc(S(=O)(=O)O)cc3C(C)(C)/C/1=C\C=C\C=C\C1=*(c3ccc(cc3C1(C)C)S(=O)(=O)[O-])CC)c(ncn2)N)O

Shipping, Storage and Handling

Shipping Conditions: Shipped on gel packs.

Storage Conditions: Store at -20 °C.

Temperature Stability: Short-term exposure to ambient temperature is possible for up to one week cumulatively.

Handling Instructions: Please centrifuge briefly before opening (volume ≤ 2 mL).

Shelf Life: 12 months after date of delivery.

Product Classification

UNSPSC: 12352208 – Nucleic acids

HS Code: 29349990

eClass Number: 34160990

eClass Product Group: Nucleic acid (not classified)

Selected References

[1] Braslavsky et al. (2003). Sequence information can be obtained from single DNA molecules.

[2] Reference details not provided.

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