ANTIBODIES / ACTIN-BINDING PROTEINS

Anti-Gelsolin/GSN Antibody Picoband® (HRP Conjugate)

HRP-conjugated antibody for gelsolin detection in biomedical and cell biology research.

Background

Gelsolin (GSN), also known as brevin, is an actin-binding protein that plays an important role in regulating the organization and dynamics of the actin cytoskeleton. It belongs to the gelsolin/villin superfamily and is involved in actin filament severing, capping and regulation of filament assembly and disassembly.

Gelsolin exists in both intracellular and extracellular forms. Intracellular gelsolin contributes to cytoskeletal remodeling, influencing cell shape, motility, adhesion and intracellular organization. These functions make it an important protein in studies of cell structure and movement.

Extracellular gelsolin participates in the actin-scavenging system, together with vitamin D-binding protein (also known as Gc protein). This system helps remove extracellular actin released following tissue injury or cell damage, limiting the potentially harmful effects of actin accumulation in the circulation and extracellular environment.

Gelsolin is also involved in the regulation of apoptosis. Experimental studies have suggested that it can influence mitochondrial pathways associated with cytochrome c release and programmed cell death. Its functions in cytoskeletal organization and cell survival make it relevant to research into inflammation, tissue injury and disease mechanisms.

Product Description

Anti-Gelsolin/GSN Antibody Picoband® (HRP Conjugate) is designed for the detection of gelsolin protein in research samples. The antibody is conjugated to horseradish peroxidase (HRP), an enzyme commonly used in antibody-based detection systems.

HRP catalyzes the conversion of suitable substrates to generate a detectable signal. This enzyme-labeling approach is widely used in immunodetection methods and can support visualization of target proteins depending on the experimental system and detection reagents.

This antibody is reported to react with Human, Monkey, Mouse and Rat samples and has been tested in the following applications:

• Western Blot (WB): Detection of gelsolin in protein extracts.
• Immunohistochemistry (IHC): Investigation of gelsolin expression in tissue sections.
• Immunocytochemistry (ICC): Detection and localization of gelsolin in cultured cells.
• Immunofluorescence (IF): Visualization of protein distribution in cellular samples.
• Flow Cytometry: Analysis of antibody-associated signals in cell populations, subject to protocol compatibility.

Key Features

• Target protein: Gelsolin (GSN).
• HRP-conjugated antibody format.
• Reported reactivity with Human, Monkey, Mouse and Rat.
• Tested applications include WB, IF, IHC, ICC and Flow Cytometry.
• Suitable for research involving actin regulation and cytoskeletal biology.

Research Applications

Gelsolin is involved in several important cellular processes, making it a useful target for a variety of research investigations.

• Actin cytoskeleton organization and remodeling.
• Cell migration, adhesion and morphology.
• Apoptosis and mitochondrial signaling research.
• Extracellular actin clearance and tissue injury studies.
• Inflammation and cellular stress research.
• Protein expression and localization analysis.

Technical Summary

Research Use

For research use only. Please consult the manufacturer's technical documentation for validated protocols, recommended working concentrations, storage conditions and handling instructions. HRP-conjugated antibody compatibility may depend on the detection method and experimental setup.