Anti-D14 Strigolactone Esterase D14 Antibody

Product Overview

Anti-D14 is a polyclonal antibody targeting strigolactone esterase D14, a protein involved in the strigolactone signaling pathway in plants. The antibody is raised in rabbit and is intended for research involving Arabidopsis thaliana.

D14 is associated with the perception and signaling of strigolactones, a class of plant hormones that contributes to the regulation of plant development and architecture. Studying D14 can help researchers investigate plant signaling mechanisms and the molecular regulation of plant growth.

This antibody is specified for Western blot analysis, with a recommended dilution of 1:5000. The supplied immunogen is a KLH-conjugated peptide derived from Arabidopsis thaliana D14.

Key Product Features

• Target protein: Strigolactone esterase D14.
• Host species: Rabbit.
• Antibody type: Polyclonal.
• Specified application: Western blot (WB).
• Recommended Western blot dilution: 1:5000.
• Species reactivity: Arabidopsis thaliana.
• Format: Lyophilized.
• Supplied quantity: 50 µL; reconstitute with 50 µL of sterile water.

Technical Specifications

Biological Background: D14 and Strigolactone Signaling

Strigolactones are plant signaling molecules involved in several aspects of plant development, including shoot branching and root-related processes. D14 functions as a key component of the strigolactone perception and signaling machinery.

Research on D14 can help clarify how plants perceive hormonal signals and translate them into molecular and developmental responses. Anti-D14 provides a research tool for examining the target protein by Western blot under appropriately validated experimental conditions.

Application: Western Blot (WB)

Western blotting is used to detect specific proteins in complex biological samples. Proteins are separated by electrophoresis, transferred to a membrane, and probed with a primary antibody directed against the protein of interest.

For this antibody, the recommended starting dilution is 1:5000. Actual working conditions may depend on sample preparation, protein abundance, transfer efficiency, detection reagents, and experimental setup. Optimize conditions as needed and consult the manufacturer's instructions.

Specificity note: The supplier indicates that this antibody recognizes recombinant AtD14. Validate performance with the intended sample and appropriate controls before interpreting experimental results.

Reconstitution and Handling

1. Briefly spin the tube before opening to collect material from the cap and sides.
2. Reconstitute the lyophilized antibody by adding 50 µL of sterile water.
3. Mix gently according to the manufacturer's instructions.
4. Prepare aliquots after reconstitution to minimize repeated freeze-thaw cycles.
5. Store the lyophilized or reconstituted antibody at −20°C, as specified by the supplier.

Reference

Yao et al. (2021). Desmethyl butenolides are optimal ligands for karrikin receptor proteins. New Phytologist. Published online January 21, 2021. DOI: 10.1111/nph.17224. PMID: 33474738.