Product Information

Product name: Anti-CD9 Rabbit Monoclonal Antibody

Catalog number: 0519-M01202

Size: 100 µL/vial

Target: CD9 (Cluster of Differentiation 9)

Antibody type: Rabbit monoclonal antibody

Reported species reactivity: Human, mouse, and rat

Reported applications: Western blot (WB), immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), immunoprecipitation (IP), and flow cytometry.

Dry ice required: No

Hazardous: No

Availability: 24/48-hour stock items; 2–6 weeks for non-stock items. Confirm current availability before ordering.

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What Is CD9?

Cluster of Differentiation 9 (CD9) is a cell-surface protein belonging to the tetraspanin family. In humans, it is encoded by the CD9 gene and is involved in organizing membrane proteins and regulating interactions between cells and their surrounding environment.

Tetraspanins are characterized by four transmembrane domains that allow them to interact with other membrane components. CD9 helps form specialized membrane microdomains, sometimes called tetraspanin-enriched microdomains, where proteins involved in cell adhesion, signaling, and membrane organization can associate.

CD9 is expressed in several cell types and has been investigated in processes such as cell adhesion, migration, fusion, and extracellular vesicle biology. Its precise function depends on the cell type, interacting proteins, and biological context.

Biological Functions of CD9

1. Cell Adhesion and Migration

CD9 contributes to the organization of cell-surface proteins involved in adhesion and interactions with neighboring cells or extracellular structures. These interactions can influence cell movement, tissue organization, and cellular responses to environmental signals.

2. Membrane Organization and Signaling

Through its interactions with other membrane proteins, CD9 helps organize molecular complexes at the cell surface. These complexes can affect signaling pathways and the activity of proteins involved in cell communication.

3. Extracellular Vesicle Research

CD9 is widely studied as a marker associated with extracellular vesicles, including many small extracellular vesicles commonly investigated in exosome research. Researchers may examine CD9 alongside other markers to characterize vesicle preparations. However, CD9 expression alone does not establish the identity, purity, or origin of an extracellular vesicle population.

4. Cell Fusion and Reproductive Biology

CD9 has been studied in membrane fusion processes, including sperm–egg interactions. Its function in these systems illustrates the importance of tetraspanins in coordinating membrane proteins and cellular interactions.

What Is an Anti-CD9 Rabbit Monoclonal Antibody?

An Anti-CD9 antibody is designed to recognize the CD9 protein and support its detection in biological samples. Monoclonal antibodies recognize a particular epitope, or molecular region, of their target, helping researchers investigate protein expression and localization under defined experimental conditions.

The Anti-CD9 Rabbit Monoclonal Antibody, catalog number 0519-M01202, is supplied in a 100 µL vial. According to the supplied product information, it has been tested in Western blot, immunohistochemistry, immunocytochemistry/immunofluorescence, immunoprecipitation, and flow cytometry applications, with reported reactivity in human, mouse, and rat samples.

Experimental performance depends on sample preparation, fixation conditions, target abundance, antibody concentration, and the detection system. Researchers should consult the manufacturer's datasheet for validated protocols and product-specific recommendations.

Research Applications of Anti-CD9 Antibody

CD9 and Extracellular Vesicle Characterization

Extracellular vesicles are membrane-enclosed particles released by cells that participate in intercellular communication. CD9 is frequently investigated as one of several proteins associated with extracellular vesicles, alongside markers such as CD63 and CD81.

Antibody-based CD9 detection can contribute to the characterization of extracellular vesicle preparations. For robust analysis, researchers should combine multiple complementary methods, such as particle analysis, protein-marker assessment, and appropriate controls, rather than relying on a single marker.

Experimental Considerations

• Confirm the antibody's suitability for the intended assay and sample type.
• Review recommended antibody dilution, incubation conditions, and sample preparation in the product datasheet.
• Use appropriate positive and negative controls to assess staining specificity.
• For flow cytometry, select a compatible detection strategy and include suitable fluorescence controls.
• For extracellular vesicle research, evaluate CD9 alongside additional markers and complementary characterization methods.
• Follow the manufacturer's storage conditions and handling recommendations.

Frequently Asked Questions

What does CD9 stand for?

CD9 stands for Cluster of Differentiation 9. It is a cell-surface tetraspanin protein involved in membrane organization and cellular interactions.

What is CD9 used as a marker for?

CD9 is studied as a cell-surface marker and is commonly used as one of several markers for extracellular vesicle characterization.

Which species are reported to react with this antibody?

The supplied product information reports reactivity with human, mouse, and rat samples. Confirm suitability for the specific sample and application before use.

Which applications have been reported for this antibody?

The supplied description lists Western blot, immunohistochemistry, immunocytochemistry/immunofluorescence, immunoprecipitation, and flow cytometry.

What is the catalog number and product size?

The catalog number is 0519-M01202, and the stated product size is 100 µL per vial.

Conclusion

CD9 is a multifunctional tetraspanin involved in cell-surface organization, cellular interactions, and extracellular vesicle research. The Anti-CD9 Rabbit Monoclonal Antibody provides a research tool for investigating CD9 protein expression across several reported experimental applications. Appropriate controls and application-specific validation are essential for reliable interpretation.

Research use only. Confirm product-specific specifications, validated applications, and storage instructions in the manufacturer's documentation before use.