Reversible capture of Twin-Strep-tag^® fusion proteins for SPR analysis

Twin-Strep-tag^® Capture Kit is intended for site-directed, reversible capture of Twin-Strep-tag^® fusion proteins for biomolecular interaction analysis using surface plasmon resonance (SPR) devices. The kit provides Strep-Tactin^®XT, Immobilization Buffer, Regeneration Buffer, and GFP-Twin-Strep-tag^® as control protein. It enables the highly stable immobilization of the ligand based on the unique Strep-Tactin^®XT:Twin-Strep-tag^® interaction, which operates with pM affinity.

The key benefits are:
• Indirect immobilization protecting the ligand from chemical modification as well as inactivation or manipulation by the immobilization procedure
• High affinity enabling measurements over a long period (high affinity in low pM range; T1/2 = 13 h)
• Chip can be regenerated easily by an established procedure – cost effective
• Ligands can be captured directly from complex samples due to the high affinity and specificity of the Twin-Strep-tag^®:Strep-Tactin^®XT interaction

The use of unconjugated Strep-Tactin^®XT requires a license that is provided with the purchase of the kit as limited use license.

Specifications

Shipping information