Description

Strep-Tactin® Buffer Set |2-1002-001

Strep-Tactin^® purification buffer set

Strep-Tactin^® Buffer Set contains all buffers necessary for purification of Strep-tag^®II or Twin-Strep-tag^® fusion proteins with Strep-Tactin^® as well as for subsequent regeneration of the resin. 10x Buffer W is a 10x concentrated wash buffer for Strep-Tactin^® and Strep-Tactin^®XT resins. It removes unbound molecules from the affinity chromatography matrix during Strep-tag^®II and Twin-Strep-tag^® fusion protein purification. 10x Buffer E is a 10x concentrated Strep-Tactin^® elution buffer. The contained desthiobiotin is a modified form of biotin that displaces Strep-tag^®II and Twin-Strep-tag^® fusion proteins from the engineered biotin-binding pocket of Strep-Tactin^® in a competitive manner, resulting in a mild elution. Please note that 10x Buffer E is not applicable for elution from Strep-Tactin^®XT resins. 10x Buffer R is a 10x concentrated Strep-Tactin^® regeneration buffer containing HABA. HABA (2-[4'-hydroxy-benzeneazo]benzoic acid) displaces desthiobiotin from the modified biotin-binding pocket of Strep-Tactin^® after the elution of Strep-tag^®II or Twin-Strep-tag^® fusion proteins. The yellow buffer turns red upon binding of HABA to Strep-Tactin^®, clearly indicating the regeneration process and activity status of the column. Please note that the Strep-Tactin^® Buffer Set is not applicable for Strep-Tactin^®XT.

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