Product Information

Product name: Anti-FBXL11/KDM2A Antibody Picoband® (PE Conjugate)

Catalog number: 0519-A03027-1-PE

Size: 100 µg/vial

Target: FBXL11/KDM2A

Conjugate: Phycoerythrin (PE)

Reported species reactivity: Human, mouse, and rat

Reported application: Western blot (WB) for the antibody described in the supplied product information. Confirm suitability for the PE-conjugated format and your intended assay before use.

Dry ice required: No

Hazardous: No

Availability: 24/48-hour stock items; 2–6 weeks for non-stock items. Confirm current availability before ordering.

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What Is FBXL11/KDM2A?

Lysine-specific demethylase 2A (KDM2A), also known as FBXL11, is a protein involved in epigenetic regulation and chromatin organization. It is encoded by the human KDM2A gene and belongs to the Jumonji C (JmjC) domain-containing histone demethylase family.

KDM2A is best known for its ability to remove methyl groups from mono- and dimethylated lysine 36 on histone H3, commonly written as H3K36me1 and H3K36me2. These chemical modifications influence chromatin structure and gene transcription. By changing histone methylation patterns, KDM2A contributes to the regulation of gene expression and cellular processes.

Biological Function of KDM2A

Histones are proteins around which DNA is organized to form chromatin. Chemical modifications to histones help determine how accessible specific regions of DNA are to transcriptional machinery. KDM2A participates in this regulatory system by modifying histone methylation marks.

KDM2A can also associate with CpG-rich regions of DNA, including CpG islands, through its DNA-binding domains. Depending on the cellular context, its activity can contribute to transcriptional repression and the maintenance of particular gene-expression patterns.

KDM2A contains a JmjC catalytic domain associated with histone demethylase activity, as well as additional domains involved in molecular recognition and protein interactions. Although its name includes FBXL11, its established role as a histone demethylase is central to research on epigenetic regulation.

What Is an Anti-FBXL11/KDM2A Antibody?

An Anti-FBXL11/KDM2A antibody is designed to recognize the KDM2A protein, supporting the detection and investigation of this target in biological samples. Target-specific antibodies are useful tools for examining protein expression, comparing experimental conditions, and studying molecular pathways involved in chromatin regulation.

The Anti-FBXL11/KDM2A Antibody Picoband® (PE Conjugate), catalog number 0519-A03027-1-PE, is supplied in a 100 µg/vial format. PE, or phycoerythrin, is a fluorescent dye commonly used in fluorescence-based detection methods. However, the appropriate assay depends on the conjugated product's validated specifications, so researchers should consult the product datasheet for confirmed applications and detection requirements.

Potential Research Applications

Understanding PE Conjugation

Phycoerythrin is a fluorescent protein commonly used as a label in fluorescence-based assays. When excited with an appropriate light source, PE emits fluorescence that can be detected using compatible optical equipment. PE conjugation is particularly common in flow cytometry and other fluorescence-based methods.

The presence of a PE label does not, by itself, establish that an antibody is suitable for every fluorescence-based technique. Researchers should verify the product's recommended application, excitation and emission requirements, sample preparation, and instrument compatibility before designing an experiment.

Experimental Considerations

• Confirm that the antibody format is appropriate for the intended assay.
• Review the manufacturer's datasheet for validated applications and recommended working conditions.
• Use suitable positive and negative controls to assess specificity.
• Follow the recommended storage conditions and protect fluorescent reagents from unnecessary light exposure.
• Optimize sample preparation and detection settings for the target and experimental system.
• Interpret results alongside appropriate controls and complementary experimental evidence.

Frequently Asked Questions

What does KDM2A stand for?

KDM2A stands for lysine-specific demethylase 2A, an enzyme involved in regulating histone methylation and gene expression.

Is FBXL11 the same as KDM2A?

Yes. FBXL11 is an alternative name for KDM2A, and both names refer to the same protein.

What is the role of KDM2A in epigenetics?

KDM2A removes methyl groups from H3K36me1 and H3K36me2, helping regulate chromatin-associated processes and gene transcription.

What does PE conjugate mean?

PE conjugate means that an antibody is linked to phycoerythrin, a fluorescent label used in compatible detection systems.

What is the catalog number and product size?

The catalog number is 0519-A03027-1-PE, and the stated product size is 100 µg per vial.

Conclusion

KDM2A/FBXL11 is an important protein in epigenetic research because of its role in histone H3 lysine-36 demethylation and transcriptional regulation. The Anti-FBXL11/KDM2A Antibody Picoband® (PE Conjugate) is a research reagent intended to support the investigation of this protein. Researchers should verify the conjugated format's validated applications and experimental requirements before use.

Research use only. Confirm product-specific specifications, validated applications, and storage instructions in the manufacturer's documentation before use.