A live cell assay for SARS-CoV-2 3CLpro enzyme activity and inhibition.

3CLpro (Mpro) Inhibitor Assay

The 3CLglow Assay kit includes 2 BacMam viruses. One expresses the 3CLpro enzyme, the other expresses a green fluorescent biosensor for 3CLpro enzyme activity as well as a red fluorescent protein to control for compound toxicity.

• Identify protease inhibitors that target SARS-CoV-2 replication
• Cost effective screening with minimal safety requirements (BSL-1)

Materials in the Kit:

• Green Fluorescent 3CLpro reporter in BacMam (3CLglow BacMam)

• 3CLpro Enzyme in BacMam

• Sodium Butyrate, 500 mM in H₂O