Autophagy Assay Kit is used for the measurement of autophagy.

Suitable Sample:

Live Cells

Excitation/
Emission (nm):

330/520 nm

Detection Method:

Fluorometric

Instrument Platform:

Fluorescence microscope, Fluorescence microplate reader

Regulation Status:

For research use only (RUO)

Storage Instruction:

Store the kit in desiccated environment at -20°C and avoid from light.

Note:

Component A-labeled vesicles are induced by starvation in HeLa cells.

Hela cells were incubated in a regular DMEM medium as control (A) or in a serum-depleted medium as autophagy treatment (B) for 16 hours. Both control cells and starved cells were incubated with component A for 30 minutes in a 37°C, 5% CO₂ incubator, and then washed four times with wash buffer. Cells were imaged immediately under a fluorescence microscope with a DAPI channel. Autophagy is indicated by bright blue dot staining of autophagic vacuoles (B).