Collagenase NB 5 Sterile Grade | N0002778

Collagenase NB 5 Sterile Grade - For Process Development

For Human ADSCs, Chondrocytes, and Stem Cell Passaging

Collagenase NB 5 Sterile Grade is a natural enzyme mixture that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease and is sterile according to European Pharmacopoeia. The balanced ratio of these activities ensures gentle and efficient tissue dissociation. Collagenase NB 5 is applicable to tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and their passaging, and rodent osteoblasts. This product is also part of our Translational Collagenase NB portfolio. It has enzymatic properties similiar to Collagenase NB 4 Standard Grade for use in research applications and Collagenase NB 6 GMP Grade for clinical applications like regenerative medicine and transplantation.

Product Information
Origin: Clostridium histolyticum
Composition: Contains class I and class II collagenase1 and a balanced ratio of proteolytic side activities
Molecular weight: 70 – 120 kDa (collagenases)
Activities: Collagenase activity: ≥ 0.10 U/mg (PZ activity* acc. to Wünsch, 25 °C)
Storage conditions: +2 °C to +8 °C
Shipping conditions: At ambient temperature worldwide
Intended use: For research use only

*PZ Unit (acc. to Wünsch2):
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.