Spermidine (SMD) ELISA Kit | abx585001

Spermidine (SMD) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Spermidine (SMD) concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Introduction: Spermidine is a polyamine compound (C7H19N3) found in ribosomes and living tissues, and having various metabolic functions within organisms. It was originally isolated from semen. Polyamines, such as spermidine, are polycationic aliphatic amines and are multifunctional. They serve vital roles in cell survival. Spermidine synthase (SPDS) catalyzes the formation of spermidine from putrescine. Spermidine is a precursor to further polyamines, such as spermine and its structural isomer thermospermine. Spermidine synchronizes an array of biological processes (such as Ca2+, Na+, K+ -ATPase) thus maintaining membrane potential and controlling intracellular pH and volume. Spermidine regulates biological processes, such as Ca2+ influx by glutamatergic Nmethyl- d-aspartate receptor (NMDA receptor), which has been associated with nitric oxide synthase (NOS) and cGMP/PKG pathway activation and a decrease of Na+,K+-ATPase activity in cerebral cortex synaptosomes.
Target: Spermidine (SMD)
Reactivity: General (All species)
Tested Applications: ELISA
Recommended dilutions: Optimal dilutions/concentrations should be determined by the end user.
Storage: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity: The validity for this kit is 6 months.
Stability: The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
Test Range: 0.78 ng/ml - 200 ng/ml
Sensitivity: < 0.29 ng/ml