The fluorescent, genetically-encoded Ca²⁺ indicator (GECO) was developed by Robert Campbell’s lab at University of Alberta. R-GECO can be paired with cADDis, our green fluorescent cAMP sensor, or our green fluorescent Upward and Downward DAG sensors to simultaneously detect both Ca²⁺ and cyclic AMP or Ca²⁺ and diacylglycerol in living cells. The BacMam delivery system enables consistent and controllable expression in a wide variety of cell types including iPSC-derived cardiomyocytes and neurons.

Materials in the Kit:

• R-GECO Ca²⁺ Sensor BacMam

• Sodium Butyrate, 500 mM in H₂O

• M₁ Muscarinic acetylcholine Receptor BacMam with nuclear green label

• Carbachol 25 mM in H₂O