Baculovirus pseudotyped with SARS-CoV-2 D614G mutated spike protein that binds with ACE2 and expresses green fluorescent protein at the nucleus 24 hours after viral entry.

Baculovirus vector pseudotyped with D614G mutated SARS-CoV-2 spike protein:

D614G Pseudo SARS-CoV-2 is a BSL-1 baculovirus  pseudotyped with D614G spike isolate. Pseudovirus enters the host cell through interactions with ACE2 and other host proteins, then delivers a reporter that expresses bright green fluorescence in the host cell nucleus. When viral entry is successfully blocked, the host fluorescence is decreased.

D614G PseudoSARS-CoV-2 is pseudotyped with the D614G variant of the SARS-CoV-2 spike protein. This European isolate spike protein decorates the surface of the pseudovirus, facilitating entry into cells expressing ACE2.

• Detect host-pathogen interaction in living cells.
• Identify therapeutic drugs that block ACE2-mediated viral entry.
• Cost effective screening with minimal safety requirements (BSL-2).

Materials in the Kit:

• D614G Pseudo SARS CoV-2 Green NLS

• Sodium Butyrate, 500 mM in H₂O

• Detailed protocol