Lipid (Oil Red O) staining Kit allows selective detection of neutral lipids within cultured cells. The kit also includes Hematoxylin to stain nuclei.

• Suitable Sample:
• Cell culture: Adipocytes, Hepatocytes, Macrophages, etc.

• Reactivity:
• Mammals

• Regulation Status:
• For research use only (RUO)

• Storage Instruction:
• Store at room temperature.

• Note:
• Result Data
• 

  Differentiated 3T3-L1 adipocytes (a) before staining with Oil Red O (b) after staining with Oil Red O. Neutral lipids are stained red and nuclei are stained blue. Oil Red O staining on undifferentiated cells (c, e) at 10X and 20X magnifications and differentiated cells (d, f) at 10X and 20X magnifications respectively. (g) Quantification of Oil Red O staining in preadipocytes and adipocytes. Cells were grown in a 24-well plate, stain was extracted in 250 uL isopropanol and 200 uL was used to measure oil Red O stain in a 96-well plate reader at 492 nm. Assay was performed according to the kit protocol.