Description

HyperLadder™ 50bp | BIO-33054

Ready-to-use molecular weight marker, designed for easy size determination of nucleic acids from 50 bp to 2000 bp.

Product Highlights

Broad size range – 15 evenly distributed bands from 50 bp to 2000 bp
Easy to orientate – several brighter reference bands
Stable – can be stored at room temperature for up to 6 months
Supplied with loading dye – for loading sample DNA

Product Description

HyperLadder™ 50bp is a molecular weight marker, especially designed for easy size determination of linear double-stranded DNA fragments on 2% to 3% TAE or TBE agarose gels.

The 10 regularly spaced bands, ranging from 50 bp to 2000 bp, with higher intensity reference bands at 300 bp, 1000 bp and 2000 bp allow for easy identification and orientation of your band at a glance.

This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 50bp from the vial to the gel. The concentration of DNA in each of the HyperLadder 50bp bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The HyperLadder 50bp is also supplied with a 5x loading dye for loading of sample DNA.

HyperLadder 50bp is perfect for size determination in techniques such as confirmation of PCR products as well as other downstream techniques.

Applications

Determination of PCR amplicon size
Confirmation of plasmid construction
Detection of DNA polymorphisms
Restriction fragment mapping

Components

Reagent	200 Lanes	500 Lanes
HyperLadder™ 50bp	2 x 500 µL	5 x 500 µL
5x Sample Loading Buffer	1 mL	1 mL

Storage & Stability

All components are shipped at room temperature or on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

Components may also be stored at +4°C or room temperature if required, although storage at -20°C is always recommended.

When stored at +4°C, reagents will remain stable for a period of 12 months.

When stored at room temperature, reagents will remain stable 6 months from date of receipt.

Shipping conditions

Ambient temperature.