Description

HyperLadder™ 25bp | BIO-33057

Ready-to-use molecular weight marker, designed for easy size determination of nucleic acids from 25 bp to 500 bp.

Product Highlights

Broad size range – 12 evenly distributed bands from 25 bp to 500 bp
Easy to orientate – several brighter reference bands
Stable – can be stored at room temperature for up to 6 months
Supplied with loading dye – for loading sample DNA

Product Description

HyperLadder™ 25bp is a molecular weight marker, especially designed for easy size determination of linear double-stranded DNA fragments on 2% to 3% TAE or TBE agarose gels or polyacrylamide gels.

The 10 regularly spaced bands, ranging from 25 bp to 500 bp, with higher intensity reference bands at 100 bp and 200 bp allow for easy identification and orientation of your band at a glance.

This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 25bp from the vial to the gel. The concentration of DNA in each of the HyperLadder 25bp bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The HyperLadder 25bp is also supplied with a 5x loading dye for loading of sample DNA.

HyperLadder 25bp is perfect for size determination in techniques that produce very short fragments such as apoptotic DNA fragmentation as well as other downstream techniques.

Applications

Apoptotic DNA fragmentation
Restriction fragment mapping
Confirmation of plasmid construction
Detection of DNA polymorphisms
Southern/Northern blotting
Cloning

Specification

Components

Reagents	200 Lanes	500 Lanes
HyperLadder™ 25bp	2 x 500 µL	5 x 500 µL
5x Sample Loading Buffer	1 mL	1 mL

Storage & Stability

All components are shipped at room temperature or on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

Components may also be stored at +4°C or room temperature if required, although storage at -20°C is always recommended.

When stored at +4°C, reagents will remain stable for a period of 12 months.

When stored at room temperature, reagents will remain stable 6 months from date of receipt.

Shipping conditions

At ambient temperature.