Description

HyperLadder™ 1kb | BIO-33053

Ready-to-use molecular weight marker with higher intensity bands for easy orientation, used for size determination of nucleic acids from 200 bp to 10 kb.

Product Highlights

Broad size range – 14 evenly distributed bands from 200 bp to 10 kb
Easy to orientate – several brighter reference bands
Stable – can be stored at room temperature for up to 6 months
Supplied with loading dye – for loading sample DNA

Product Description

HyperLadder™ 1kb is our most popular molecular weight marker, composed of a restriction digest plus one or more PCR products, especially designed for easy size determination of linear double-stranded DNA fragments on 1% to 2% TAE or TBE agarose gels.

The 14 regularly spaced, easy to remember bands, ranging from 200 bp to 10,037 bp, with higher intensity reference bands at 1000 bp and 10,037 bp and a doublet band at 1500/1517 bp allow for easy identification and orientation of your band at a glance.

The ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 1kb from the vial to the gel. The approximate mass of DNA in each of the HyperLadder 1kb bands is provided for optionally determining the approximate mass of DNA in comparably intense samples of similar size. The HyperLadder 1kb is also supplied with a 5x loading dye for loading of sample DNA.

With a wide size range, HyperLadder 1kb is perfect for size determination in techniques such as sequence analysis, confirmation of plasmid construction and PCR products as well as Southern blotting and other downstream techniques.

Applications

Restriction fragment mapping
Sequence analysis
Confirmation of plasmid construction
Detection of DNA polymorphisms
Southern/Northern blotting
Cloning

Specification

Components

Reagent	200 Lanes	500 Lanes
HyperLadder™ 1kb	2 x 500 µL	5 x 500 µL
5x Sample Loading Buffer	1 mL	1 mL

Storage & Stability

All components are shipped at room temperature or on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

Components may also be stored at +4°C or room temperature if required, although storage at -20°C is always recommended.

When stored at +4°C, reagents will remain stable for a period of 12 months.

When stored at room temperature, reagents will remain stable 6 months from date of receipt.

Shipping conditions

Ambient temperature.