Description

HyperLadder™ 100bp | BIO-33056

Ready-to-use molecular weight marker, designed for easy size determination of nucleic acids from 100 bp to 1 kb.

Product Highlights

Broad size range – 10 evenly distributed bands from 100 bp to 1 kb
Easy to orientate – several brighter reference bands
Stable – can be stored at room temperature for up to 6 months
Supplied with loading dye – for loading sample DNA

Product Description

HyperLadder™ 100bp is a molecular weight marker, composed of a restriction digest plus one or more PCR products, especially designed for easy size determination of linear double-stranded DNA fragments on 1% to 2% agarose gels.

The 10 regularly spaced bands, ranging from 100 bp to 1013 bp, with a doublet band at 300/311 bp and higher intensity reference bands at 1013 bp allow for easy identification and orientation of your band at a glance.

This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 100bp from the vial to the gel. The concentration of DNA in each of the HyperLadder 100bp bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The HyperLadder 100bp is also supplied with a 5x loading dye for loading of sample DNA.

HyperLadder 100bp is perfect for size determination in techniques such as confirmation of plasmid construction and PCR products as well as other downstream techniques.

Applications

Restriction fragment mapping
Determination of PCR amplicon size
Confirmation of plasmid construction
Detection of DNA polymorphisms
Southern/Northern blotting
Cloning

Specification

Components

Reagent	200 Lanes	500 Lanes
HyperLadder™ 100bp	2 x 500 µL	5 x 500 µL
5x Sample Loading Buffer	1 mL	1 mL

Storage & Stability

All components are shipped at room temperature or on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

Components may also be stored at +4°C or room temperature if required, although storage at -20°C is always recommended.

When stored at +4°C, reagents will remain stable for a period of 12 months.

When stored at room temperature, reagents will remain stable 6 months from date of receipt.

Shipping conditions

Ambient temperature.

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