Glucose Uptake Assay Kit (Red Fluorescence) is used for the measurement of glucose uptake.

Suitable Sample:

Live Cells

Excitation/
Emission (nm):

540/590 nm

Detection Method:

Fluorometric

Instrument Platform:

Microplate Readers

Regulation Status:

For research use only (RUO)

Storage Instruction:

Store the kit in desiccated environment at -20°C and avoid from light.

Note:

Measurement of 2DG uptake in differentiated 3T3-L1 adipocytes and 3T3-L1 fibroblasts.

Assays were performed with Glucose Uptake Assay Kit (Red Fluorescence) (Cat # KA4085) in a black wall/clear bottom cell culture Poly-D lysine plate using a Gemini (Molecular Devices) microplate reader. (A: Negative Control, no insulin no 2DG treatment. B: 2DG uptake in the absence of insulin. C: 2DG uptake in the presence of 1 uM insulin. D: 2DG uptake in the presence of 1 uM insulin and 200 uM phloretin. E: 2DG uptake in the presence of 1 uM insulin and 5mM D-Glucose.) (Please refer to the protocol for detailed operations).