Applications: ELISA
Specificity:  dog proAKAP4 (all breeds)
Analytical range: 0 to 150 ng
Sample type: Fresh, chilled and frozen spermatozoa / straw

The Dog 4MID® Kit (Ref.4VDX-18K5) is the first immunoassay that validates objectively semen  quality by quantifying a protein biomarker named proAKAP4 in dog semen.

This ELISA kit is the 1st functional test to evaluate sperm quality in dogs (all breeds).

The dosage of the proAKAP4 concentration with the Dog 4MID® Kit will allow :

• Qualification of male ejaculates before and after cryopreservation
• Qualification of each breeding male
• Selection of the best semen for cryopreservation
• Selection of semen with long lasting motility and functionality at post-thaw
• Selection of semen with more mature, normal and less-tail abnormalities (indicator of good quality spermatogenesis 
• Selection of best extender for semen preservation

The concentration of the proAKAP4 marker is an objective indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient overtimes (Sergeant et al. 2019 ; Bastan and Akay, 2021).

High concentrations of proAKAP4 help select semen with long lasting motility and are indicative of good spermatogenesis, giving more mature, more “normal’ spermatozoa and less tail abnormalities ratio in bull semen samples (Bastan and Akcay, 2021).

Technical data about the Dog 4MID® Kit :

• Name: Dog 4MID® Kit
• Reference : 4VDX-18K5
• Specificity : dog proAKAP4 (all breeds)
• ELISA Kit (96 wells microplate)
• This ELISA kit allow to assess from 1 to 88 samples simultaneously or individually 12 breakable strips of 8 wells per plate/kit)
• Working with fresh ejaculate, chilled or frozen semen in extenders / isolated spermatozoa
• Easy to use immunoassay
• Easy to interpret / to implement in clinics and breeding centers
• Robust, objective and reproductible method

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.