CleanDTR | CDTR-0005

BigDye terminator removal sequencing clean up kit

CleanDTR is an efficient paramagnetic bead-based system, designed bij CleanNA to remove unincorporated dye terminators from Sanger sequencing reactions. It can also be used for CRISPR-Cas9. The CleanDTR process involves three simple steps including bind, wash and elute. While binding the sequencing product selectively to the magnetic particles, unincorporated dyes, nucleotides, salts and primers will be removed during ethanol washes. This principle allows for elution of the pure Sanger Sequencing product in the elution buffer of choice. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.

Applications
Clean up of sequencing product for both
ABI and MegaBACE platforms
Supported Chemistries
• BigDye* versions 1.0, 1.1, 2.0, 3.0 and 3.1
• DYEnamic ET

Features
Long Phred 20 read lengths averaging over 800 bps
Pass rates over 85% or higher
Efficient elimination of sequencing reaction contaminants
Reduce BigDye* usage, due to increased average signal strenght