Applications: ELISA
Specificity: Bull proAKAP4 (all breeds)
Analytical range: 9 to 150 ng
Sample type: Fresh, chilled or frozen semen / Isolated spermatozoa

Innovative immunoassay to assess semen quality in bulls (all breeds)     

The Bull 4MID® Kit (Ref. 4VDX-18K4) is the first immunoassay that validates objectively semen quality by quantifying a protein biomarker named proAKAP4 in samples of bull semen. 

This ELISA kit is the 1st functional test to evaluate sperm quality and fertility, as high levels of AKAP4 has been shown in high fertile bulls (Peddinti et al. 2008 : Singh et al. 2019 ; Ruelle et al. 2019 ; Ruelle et al. 2020 ; Bastan and Akcay, 2021 ; Marques de Almeida, 2022 ; Dordas-Perpinya et al. 2022).

The dosage of the proAKAP4 biomarker with the Bull 4MID® Kit, will bring :

• Objective information on semen quality in bulls (all breeds)

• Qualification of each breeding male and their ejaculates overtimes

• Selection of semen with long-lasting motility at post-thaw

• Pertinent parameter to improve sperm cryopreservation

• Selection of semen with less tail abnormalities 

• A functional indicator correlated to fertility 

The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient overtimes (Sergeant et al. 2019 ; Bastan and Akcay, 2021).

High concentrations of proAKAP4 help select semen with long lasting motility and are indicative of good spermatogenesis, giving more mature, more “normal’ spermatozoa and less tail abnormalities ratio in bull semen samples (Bastan and Akcay, 2021).

Technical data of the Bull 4MID® Kit

• Name: Bull 4MID® Kit 
• Reference : 4VDX-18K4
• Specificity : bull proAKAP4 (all breeds)
• This ELISA kit allow to assess from 1 to 88 samples simultaneously or individually (12 strips of 8 wells per microplate 96 wells / per kit)
• Working with ejaculate, chilled or frozen semen 
• Easy to use immunoassay
• Easy to interpret / implement
• Objective, robust and reproductible method

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.