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1 | PEB (4x) | protein extraction buffer | AS08 300
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Description
1 | PEB (4x) | protein extraction buffer | AS08 300
Extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection in denatured conditions.
Quantity: 5 x 2 ml (4x stock) allows up to 75 isolations of plant material (using 500 µl 1x PEB for 100 mg fresh weight) or 190 isolations of algal material (using 200 µl 1x PEB for cell amounts corresponding to 4-10 µg total chlorophyll)
Storage: Stable at RT for at least 1 month; short-term storage (6 monthss) at 4°C and long term storage (1 year or more) at -20°C.
Tested applications: protein extraction
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