iScript™ IV TaqProbe One-Step RT-qPCR Kit | D046

iScript™ IV TaqProbe One-Step RT-qPCR Kit is a complete qPCR system containing all necessary reagents for both reverse transcription and TaqMan Probe based qPCR amplification to occur in a single reaction qPCR reaction tube. RT-qPCR Enzyme Mix (10X) contains iScript™ IV Reverse Transcriptase (RT), Hot-Start Taq DNA Polymerase and RNase Inhibitor for highly sensitive and specific RT-PCR using any RNA template. Our proprietary RT-qPCR Master Mix (5X) contains stabilizers and enhancers that optimize the two reactions in a real-time “single step”. This One-Step RT-qPCR Kit offers the end-users an efficient and reliable alternative to conventional “two-step” sequential RT-qPCR. Gene-specific primers must be used along with this kit.

Applications

• Gene-expression analysis.
• Transcription analysis.
• Gene cloning.
• Virus detection and quantification.