Product Highlights

• Higher Performance - robust polymerase
• Chemical hot-start PCR enzyme - eliminates non-specific binding
• Reaction requires activation step - allows convenient reaction set-up at room temperature
• Suitable for TA cloning - leaves ´A´ overhang

Product Description

IMMOLASE™ is a heat-activated thermostable DNA polymerase and provides high yield and improved specificity when compared to standard polymerases. IMMOLASE can eliminate the presence of non-specifics such as primer-dimers and mis-primed products.

IMMOLASE is inactive at room temperature and requires activation by heat treatment for 10 minutes prior to PCR cycling. This provides greater flexibility in reaction set ups and allows the premixing of PCR reagents at room temperature. Subsequently, the reaction can be handled according to preferred protocols for thermostable DNA polymerases.

Applications

• For multiplex reactions
• Products suitable for TA cloning

Components

Concentration

5 u/µL

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions

Shipped on Dry Ice or Blue Ice.