Description
BIOTAQ™ DNA Polymerase | BIO-21040
BIOTAQ is a highly purified, thermostable DNA polymerase offering high yield over a wide range of PCR templates and a good choice for routine PCR assays.
BIOTAQ is a robust preparation and delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.
Product Highlights
- Good standard Taq polymerase - ideal for setting up new procedures
- Easy to use - designed for easy optimization of PCR applications
- Suitable for TA cloning - leaves 'A' overhang
Product Description
BIOTAQ™ is a purified thermostable DNA polymerase offering high yield over a wide range of PCR templates, and is a good choice for routine assays. BIOTAQ is a robust preparation and delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.
BIOTAQ is supplied with 10x NH4-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl2 is provided to allow reaction conditions to be adjusted to suit the template.
Applications
- Routine PCR applications
- TA cloning
Specification
Components
|
Reagent |
500 Units |
2500 Units |
|
BIOTAQ DNA Polymerase |
1 x 100 µL |
5 x 100 µL |
|
10x NH4 Reaction Buffer |
2 x 1.2 mL |
10 x 1.2 mL |
|
50 mM MgCl2 Solution |
1 x 1.2 mL |
5 x 1.2 mL |
Concentration
5 u/μL
Storage & Stability
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
Shipping conditions
On Dry Ice or Blue Ice.
One unit will incorporate 10 nmoles of dNTPs in 30 min at 72°C.
BIOTAQ is a trademark of Meridian.
Related Products
IMMOLASE™ DNA Polymerase | BIO-21046
Gentaur
ACCUZYME™ DNA Polymerase | BIO-21052
Gentaur
Taq DNA Polymerase | D006
Gentaur
MagniPhi® DNA Polymerase | 500025
Gentaur
QualiPhi® DNA Polymerase | 510025
Gentaur
HotStart Taq DNA Polymerase | D007
Gentaur
nanoTaq Hot-Start DNA Polymerase | DP001-0100
Gentaur
Uracil DNA Glycosylase | BIO-27044
Gentaur
