Intracellular Total ROS Activity Assay Kit (Orange Fluorescence) is used for the measurement of intracellular total ROS activity.

Suitable Sample:

Live Cells

Excitation/
Emission (nm):

540/570 nm

Detection Method:

Fluorometric

Instrument Platform:

Flow Cytometer, Fluorescence Microplate Reader, Fluorescence Microscope

Regulation Status:

For research use only (RUO)

Storage Instruction:

Store the kit in desiccated environment at -20°C and avoid from light.

Note:

Detection of ROS in Hela cells.

Top: Hela cells were seeded overnight at 15,000 cells/90uL/well in a Costar black wall/clear bottom 96-well plate. The cells were untreated (control) or treated with1 mM H₂O₂ or 100 uM tert-butyl hydroperoxide (TBHP) for 30 min at 37°C. The ROS 570 assay solution (100uL/well) was added and incubated in a 5% CO₂, 37°C incubator for 1 hour. The fluorescence signal were monitored at Ex/Em = 540/570 nm (cut off = 550 nm) with bottom read mode using FlexStation (Molecular Devices).
Bottom: Images of Hela cells stained with the Intracellular Total ROS Activity Assay Kit (Orange Fluorescence) (Cat # KA4075) in a Costar black wall/clear bottom 96-well plate. A: Untreated control cells. B: Cells treated with 100 uM tert-butyl hydroperoxide (TBHP) for 30min before staining.