Description

ImmoMix™ Red | BIO-25022

ImmoMix™ is a complete ready-to-use, red-colored, high yield; heat-activated, 2x reaction-mix which simply requires the user to add water, template and primers, and then pre-heat to 95°C for 10 minutes to carry out successful PCR assays. Once completed, the reaction mix can be loaded directly on to a gel.

Product Highlights

Convenient - pre-mixed, pre-optimized 2x solution
Ready to use format - reduces risk of contamination
Fast set up - decreased time compared to traditional methods
Red color - indicates the mix has been added
Direct gel loading - no further processing necessary

Product Description

ImmoMix™ Red is a complete ready-to-use heat-activated 2x reaction-mix, which simply requires the user to add only water, template and primers, and then pre-heat to 95°C for 10 minutes to successfully carry out PCR assays. The 10 minute activation step eliminates the presence of non-specifics such as primer-dimers and mis-primed products, since the enzyme is inactive at initial low temperatures.

ImmoMix Red combines all of the features and advantages of ImmoMix and contains an additional inert red dye. This non-toxic, non-hazardous red dye allows users to load samples directly onto a gel, without the need to add loading buffer since the mix is of sufficiently high density to sink to the bottom of the gel.

Adequate mixing is also ensured when reactions are set up. The red dye migrates like a 350 bp fragment on a 2% agarose TAE gel (or 600 bp on a 1% agarose).

ImmoMix Red is based on IMMOLASE™ DNA Polymerase, which leaves an ´A´ overhang and can be used for a wide variety of templates. An additional 50 mM MgCl₂ solution is included should any fine adjustments be required.

ImmoMix Red reduce the time needed to set up reactions, thereby reducing the risk of contamination. Greater reproducibility is ensured, by reducing the number of pipetting steps that can lead to pipetting errors.

Applications

Ultra-high specificity for multiplex reactions
Products suitable for TA cloning

Components

Reagent	500 Reactions
ImmoMix Red	10 x 1.25 mL
50 mM MgCl₂ Solution	1.2 mL

Volume

BIO-25022: 500 x 50 µL Reactions 10 x 1.25 mL

Concentration

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Components may also be stored at +4°C and will remain stable for up to 2 weeks, however storage at -20°C is recommended.

Shipping conditions

On Dry Ice or Blue Ice.

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