Product Highlights

• Convenient - pre-mixed, pre-optimized 2x solution
• Ready to use format - reduces risk of contamination
• Fast set up - decreased time compared to traditional methods
• Reproducible results - time after time

Product Description

ImmoMix™ is a complete ready-to-use high yield, heat-activated 2x reaction-mix, which simply requires the user to add water, template and primers, and then pre-heat to 95°C for 10 minutes to successfully carry out PCR assays. The 10 minute activation step eliminates the presence of non-specifics such as primer-dimers and mis-primed products, since the enzyme is inactive at initial low temperatures.

ImmoMix is based on IMMOLASE™ DNA Polymerase, which leaves an ´A´ overhang, and has been optimized for a wide variety of templates. Additional MgCl₂ solution is included should any fine adjustments be required.

ImmoMix reduces the time needed to set up reactions, thereby reducing the risk of contamination. Greater reproducibility is ensured by reducing the number of pipetting steps that can lead to pipetting errors.

Applications

• Ultra-high specificity for multiplex reactions
• Products suitable for TA cloning

Components

Volume

• BIO-25020: 500 x 50 µL Reactions: 10 x 1.25 mL

Concentration

• 2x

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

Components may also be stored at +4°C if required, although storage at -20°C is recommended. When stored at +4°C, reagents will remain stable for a period of two weeks from date of receipt.

Shipping conditions

On Dry Ice or Blue Ice.