Description

BioMix™ Red | BIO-25006

BioMix™ Red is a complete ready-to-use 2x reaction mix containing an ultra-stable Taq DNA polymerase. It contains an additional inert red dye that permits easy visualization and direct loading onto a gel.

Product Highlights

Convenient format - pre-mixed, pre-optimized 2x solutions
Versatile - suitable for routine PCR applications
Ready-to-use - reduces contamination risks
Suitable for TA cloning - leaves 'A' overhang
Direct gel loading - eliminates the need for further processing following reaction completion

Product Description

BioMix™ Red is a complete ready-to-use 2x reaction mix containing a stable Taq DNA polymerase. It contains an additional inert red dye that permits easy visualization and direct loading onto a gel. There is no need to add loading buffer as the mix is of sufficiently high density to sink to the bottom of the gel. The red dye migrates like a 350 bp fragment on a 2% agarose TAE gel (or 600 bp on a 1% agarose).

BioMix Red has been developed to perform PCR assays of many common genomic and cDNA templates; the user has simply to add water, template and primers. It reduces the time required to set-up reactions, thereby minimizing the risk of contamination. Reproducibility is ensured by reducing the number of pipetting steps that can lead to errors.

BioMix Red is supplied with additional MgCl₂ solution should any fine adjustments be required.

Applications

Routine PCR applications
TA cloning
High throughput

Specification

Components

Reagent	100 Reactions	500 Reactions
BioMix™ Red	2 x 1.25 mL	10 x 1.25 mL
50 mM MgCl₂ Solution	1.2 mL	1.2 mL

Volume

BIO-25005: 100 x 50 µL Reactions: 2 x 1.25 mL
BIO-25006: 500 x 50 µL Reactions: 10 x 1.25 mL

Concentration

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Alternatively, BioMix Red can be stored for up to up to 2 weeks at +4°C.

Shipping conditions

On Dry Ice or Blue Ice.

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