Anti-GST Taq Mouse Monoclonal

Description

Affinity purified monoclonal Anti-GST-Tag, clone GST.B6, mouse IgG2a. Recognizes native and denatured forms of purified GST or GST fusion proteins. Applications: Western Blot (1:1000-3000), Dot Blot, ELISA, Immunocytochemistry (1:500-2000), Immunoprecipitation. Optimal dilutions/concentrations should be determined by the end user.

Host Species/Expression System

Mouse

Applications

WB, ICC, IP, ELISA

Clonality

clone GST.B6

Isotype

IgG2a

Storage/Stability

Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.

Shipping Temperature

-20°C or-80°C

Background

GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

Format

Aqueous buffer solution

Formulation

1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3

Species Reactivity

Schistosoma japonicum. Recognizes native and denatured forms of purified GST or GST fusion proteins. Predicted molecular weight of Schistosoma japonicum GST: 26 kDa.

Cross Reactivity

Works in human cells; predicted to work in all proteins containing the GST tag, regardless of species. Does not cross-react with mammalian GST.

Purification

Protein A Affinity Chromatography

Immunogen

Purified Schistosoma japonicum GST fusion protein.