$373.00

cPK
[GLO117-100]

Source: Bovine kidney.

Purity: > 90% by SDS-PAGE, apparent Mr ~ 45-kDa.

Supplied: 0.1 μg (~ 2 units) in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 50% glycerol.

Activity: ~ 17,000 units/mg with protamine sulfate as substrate. One unit is the amount of cPK that incorporates 1 nmol of phosphate into protamine sulfate per min. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.

Synonyms: Cytosolic Protamine Kinase; cPK

Background: Cytosolic Protamine Kinase (cPK) is a distinct insulin-stimulated protein kinase which phosphorylates eIF4E (GLO118-005, GLO118-015, GLO118-030), hnRNP protein A1, and numerous other cellular proteins. cPK is itself regulated by reversible phosphorylation. Phosphorylation by Myelin Basic Protein Kinases MBPK-1 (GLO113-100) and MBPK-2 (GLO114-100) activates cPK. Dephosphorylation by protein phosphatases PP2A1 (GLO130-001, GLO130-005), PP2A2 (GLO131-001, GLO131-005) and PP2AC (GLO132-001) inactivates the kinase.

References:

  1. J Biol Chem 264: 6412
  2. J Biol Chem 268: 15298
  3. Biochem J 287: 1019

Related Products: cPK Assay Kit ♦ Protamine Sulfate ♦ eIF4E (5 μg) ♦ eIF4E (15 μg) ♦ eIF4E (30 μg)

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