MBPK-1, Myelin Basic Protein Kinase 1
Source: Bovine kidney.
Purity: > 90% by SDS-PAGE, apparent Mr ~ 40-kDa.
Supplied: 100 ng in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 50% glycerol.
Activity: ~ 300 units/mg with myelin basic protein (MBP) as substrate. One unit is the amount of MBPK-1 that incorporates 1 nmol of phosphate into MBP per min. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Synonyms: Myelin Basic Protein Kinase 1; MBPK-1
Background: MAP kinases are important components of signal transduction pathways that regulate numerous cellular processes including cell growth, division and differentiation. MAP kinases undergo phosphorylation on specific tyrosine and threonine residues mapping within a characteristic Thr-Glu-Tyr motif. This dual phosphorylation is catalyzed by MEK in vitro and in vivo. MBPK-1 and MBPK-2 are distinct members of the MAP kinase family. They phosphorylate and activate cPK, a distinct insulin-stimulated protein kinase.
Figure: SDS-PAGE pattern of purified preparations of MBPK-1 (lane 1) and MBPK-2 (lane 2). The gel was stained with Coomassie Blue.
Reference: J Biol Chem 268: 15298