GloboZymes Anti-PAK is an affinity-purified polyclonal rabbit antibody. It was produced to a synthetic peptide corresponding to residues 484-502 of human PAK1. The antibody is useful for Western blotting. For example, the antibody may be used at 1:1000 dilution with 30 μg of HEK293 cell extracts. In addition to PAK1, the antibody displays some reactivity with PAK2.

Synonyms: Anti-p65PAK; Anti-p68PAK; Antibody to p21-Activated Protein Kinase.

Western Blot of HEK293 Extract with Ani-PAK

Figure: An extract of HEK293 cells (30 μg) was subjected to Western blotting  with GloboZymes affinity-purified Anti-PAK polyclonal antibody. The band corresponds to PAK65 (65 kDa).

Background: The p21-GTPase-activated protein kinase family (PAK’s) regulate numerous processes. These processes include cell morphology and cytoskeleton organization, cell motility, apoptosis, neuronal functions, and cell cycle progression among other processes. PAK’s interact with several important regulatory proteins including Protein Phosphatase 2A (PP2A), ribosomal protein S6 kinase, Mixed Lineage Kinase (MLK2), and the SH3-SH2 adapter Nck. Physiological targets of PAK include p38 MAP kinase, JNK, cRaf-1 and Synapsin I. The catalytic domain of PAK is produced in apoptotic cells via a caspase-dependent pathway. Unlike the native enzymes, the catalytic domain is not affected by Rac or Cdc42.

References: Nature 367: 40; J Biol Chem 270: 21695



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