GloboZymes Provides You Top Quality Products

Introduction

GloboZymes is a modern company with a highly-skilled signal transduction research team. Over the past nineteen years, GloboZymes has established itself as a leading provider of top-quality products, some of which are unique to our product line.

Below are just a few examples of some of our high quality and dependable products:

Effect of I1PP2A (Panel A) and I2PP2A (Panel B) on Protein Phosphatases. The activities (0.005 unit) of PP2A1 (closed circles), PP1C (open circles), PP2B (closed triangles), and PP2C (open triangles) were measured with 32P-labeled MBP as substrate in the presence of the indicated concentrations of purified I1PP2A and I2PP2A. Assays were performed in the absence of Mn2+ as described. Note: I1PP2A and I2PP2A were discovered as inhibitors of Protein Phosphatase 2A by GloboZymes founder Dr. Zahi Damuni.

Nuclear Localization of I2PP2A/SET in HEK293 cells. Confluent HEK293 cells, coated with poly-D-lysine and fixed onto sterile glass cover slips, were permeablized and incubated with bovine serum albumin. The albumin was removed and the cells were incubated with Lamin B Monoclonal antibody 101-B7 (panel A) or GloboZymes Anti-I2PP2A (panel B). After washing, cells were incubated with goat anti-mouse IgG (H+L) conjugated to Lissamine Rhodamine (panel A) or goat anti-rabbit IgG (H+L) conjugated to Cy2. Cover slips were washed with PBS, mounted onto glass slides, and viewed using a fluorescence microscope. Panels A and B show the cells stained with #Lissamine Rhodamine and Cy2, respectively. Panel C shows Panels A and B superimposed. Control cells probed with the secondary antibody or with preimmune serum and the secondary antibody displayed no reaction (not shown).

Figure: Effect of I2PP2A and PP2AC expression on AP-1 DNA binding.  (A) Nuclear extracts (3 ug) from cells transfected with pcDNA3.1(+­) (lane 1), pcDNA3.1(­+)/I2PP2A (lane 2) and pCMV/PP2A (lane 3) were incubated with 30 pg of 32P-labelled AP-1 probe and subjected to electrophoretic mobility-shift analysis as described. An autoradiogram of the dried gel is shown. (B) Nuclear extracts from cells transfected with pcDNA3.1(+­)/I2PP2A were incubated with the 32P-labelled AP-1 probe (as described) in the absence (lane 1) or presence of a 50-fold (lane 2) or 100-fold (lane 3) molar excess of unlabelled wild-type AP-1 probe. In lane 4, mutant probe was included at a 100 molar excess. The mixtures were subjected to electrophoretic mobility shift analysis, and the gel was dried. An autoradiogram of the gel is shown. (C) Nuclear extracts from I2PP2A-expressing cells were incubated in the absence (lane 1) or presence (lane 2) of c-Jun antibody for 60 min at room temperature, and then with the 32P-labelled probe as described. The mixtures were subjected to polyacrylamide gel electrophoresis. An autoradiogram of the dried gel is shown. 

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