Source: Recombinant human.
Purity: > 90% by SDS-PAGE, apparent Mr ~ 65-kDa.
Supplied: 5 μg in 50 μl of 50 mM Tis-HCl pH 7.0 buffer containing 0.1 mM phenylmethanesulfonyl fluoride, 14 mM β-mercaptoethanol, 1 mM EDTA and 50% glycerol. Maintain in aliquots at -70°C. Avoid repeated thawing.
Synonyms: Regulatory A 65 kDa Subunit of Protein Phosphatase 2A; PR65; A/PR65
Background: The A (PR65) subunit of protein phosphatase 2A (PP2A, GLO130-GLO132) serves as a scaffolding molecule to coordinate the assembly of the catalytic (PP2AC, GLO132-001) and variable B subunits, generating functionally diverse heterotrimers of the phosphatase. The A subunit contains 15 tandemly-repeated HEAT sequences which may participate in its function as a scaffolding molecule. The A subunit also serves to regulate the substrate selectivity and allosterically modulates the enzymatic properties of PP2AC, namely via its polycation response. Association of recombinant A subunit with PP2AC is relatively tight (apparent KD ~ 85 pM) and leads to marked suppression of phosphatase activity with several phosphoproteins substrates. By contrast, with several less-structured or smaller substrates, such as phosphopeptides, suppression of PP2A activity is markedly less. The affinity of the A subunit for PP2AC is diminished by almost 100-fold following introduction of a glutamate at position 416, indicating that the basic motif in this region of the protein may be important for its interaction with PP2AC. Some evidence indicates a role for the A subunit in disease. For example, at least four low frequency mutations in genes encoding the A subunit with altered affinity for PP2AC have been identified in several different human cancers. Additionally, overexpression of the A subunit in rat embryo fibroblasts leads to multinucleated cells.
Product Datasheet (pdf)
Related Products: A Subunit (1 μg) ♦ AGlu416 Subunit (1 μg) ♦ AGlu416 Subunit (5 μg) ♦ PP2A1 (1 μg) ♦ PP2A1 (5 μg) ♦ PP2A2 (1 μg) ♦ PP2A2 (5 μg) ♦ PP2AC (1 μg)