Source: Recombinant human.

Purity: > 90% by SDS-PAGE, apparent Mr ~ 65-kDa.

Supplied: 5 μg in 50 μl of 50 mM Tis-HCl pH 7.0 buffer containing 0.1 mM phenylmethanesulfonyl fluoride, 14 mM β-mercaptoethanol, 1 mM EDTA and 50% glycerol. Maintain in aliquots at -70°C. Avoid repeated thawing.

Synonyms: Inactive Mutant Regulatory 65 kDa A Subunit of Protein Phosphatase 2A; A^Glu416; PR65^Glu416

Background: The A^Glu416 (PR65^Glu416) subunit of protein phosphatase 2A (PP2A, GLO130-GLO132) contains a mutation in a basic motif that is an important determinant for the interaction of the A (GLO138-001, GLO138-005) and catalytic (GLO132-001) subunits of the phosphatase. GloboZymes A^Glu416 preparations can be used in reconstitution studies to further delineate the functional significance of the A subunit and its basic motif.

Product Datasheet (pdf) 

References:

• Eur J Biochem 267: 5209
• Eur J Biochem 248: 200

Related Products: A^Glu416 Subunit (1 μg)  ♦  A Subunit (1 μg)  ♦  A Subunit (5 μg)  ♦  PP2A₁ (1 μg)  ♦  PP2A₁ (5 μg)  ♦  PP2A₂ (1 μg)  ♦  PP2A₂ (5 μg)  ♦  PP2A_C (1 μg)