Source: Recombinant human kindey produced in E. coli.

Purity: > 90% by SDS-PAGE, apparent Mr ~ 30-kDa.

Supplied: 1 μg in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA, 0.1% Brij-35 and 10% glycerol. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.

Synonyms: Protein Phosphatase 2A Inhibitor Protein 1; I₁^PP2A; I₁^PP2A(a); PHAP-I; p32/33; LANP

Background: I₁^PP2A, also termed I₁^PP2A(a), PHAP-I, p32/33 and LANP, is highly expressed in some cancers. Purified preparations of this oncoprotein inhibit all forms of protein phosphatase 2A (PP2A, GLO130-GLO132) tested to date in a substrate-selective manner. The purified preparations inhibit PP2A potently (k_i ~ 0.1 - 0.5 nM) with myelin basic protein (MBP, GLO126-010, MGLO126-025) and histone H1 but not with casein as substrate. In contrast, the preparations stimulate PP1 (GLO133-001) in a substrate-selective manner. I₁^PP2A undergoes phosphorylation on serines and tyrosines in cells. The functional significance of the serine phosphorylation remains to be determined. Tyrosine phsophorylation of I₁^PP2A occurs in response to the antiproliferative lectin, Jacalin and causes the dissociation of I₁^PP2A from PP2A and the activation of the phosphatase.

Product Datasheet (pdf)

References:

1. Biochemistry 35: 6998
2. Biol Chem Hoppe-Seyler 375: 113
3. J Biol Chem 265:13400

Related Products:  I₁^PP2A GST (1 μg)  ♦  I₁^PP2A GST (5 μg)  ♦ Anti-I₁^PP2A (50 μg)  ♦  Anti-I₁^PP2A (100 μg)  ♦  I₁^PP2A Detection Kit  ♦  I₂^PP2A  ♦  I₂^PP2A GST (1 μg)  ♦  I₂^PP2A GST (5 μg)  ♦  I₂^PP2A(β) ♦  PP2A₁ ♦  PP2A₂ ♦  PP2A_C ♦  PP1_C ♦ MBP (10 mg)  ♦ MBP (25 mg)