Source: Recombinant human kindey produced in E. coli.
Purity: > 90% by SDS-PAGE, apparent Mr ~ 30-kDa.
Supplied: 1 μg in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA, 0.1% Brij-35 and 10% glycerol. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Synonyms: Protein Phosphatase 2A Inhibitor Protein 1; I1PP2A; I1PP2A(a); PHAP-I; p32/33; LANP
Background: I1PP2A, also termed I1PP2A(a), PHAP-I, p32/33 and LANP, is highly expressed in some cancers. Purified preparations of this oncoprotein inhibit all forms of protein phosphatase 2A (PP2A, GLO130-GLO132) tested to date in a substrate-selective manner. The purified preparations inhibit PP2A potently (ki ~ 0.1 - 0.5 nM) with myelin basic protein (MBP, GLO126-010, MGLO126-025) and histone H1 but not with casein as substrate. In contrast, the preparations stimulate PP1 (GLO133-001) in a substrate-selective manner. I1PP2A undergoes phosphorylation on serines and tyrosines in cells. The functional significance of the serine phosphorylation remains to be determined. Tyrosine phsophorylation of I1PP2A occurs in response to the antiproliferative lectin, Jacalin and causes the dissociation of I1PP2A from PP2A and the activation of the phosphatase.
Product Datasheet (pdf)
- Biochemistry 35: 6998
- Biol Chem Hoppe-Seyler 375: 113
- J Biol Chem 265:13400
Related Products: I1PP2A GST (1 μg) ♦ I1PP2A GST (5 μg) ♦ Anti-I1PP2A (50 μg) ♦ Anti-I1PP2A (100 μg) ♦ I1PP2A Detection Kit ♦ I2PP2A ♦ I2PP2A GST (1 μg) ♦ I2PP2A GST (5 μg) ♦ I2PP2A(β) ♦ PP2A1 ♦ PP2A2 ♦ PP2AC ♦ PP1C ♦ MBP (10 mg) ♦ MBP (25 mg)