PDC (10 μg)

Source: Bovine heart

Purity: > 90% by SDS-PAGE, subunit apparent Mr's ~ 74,000 (E2), 53,000 (E3), 41,000 (E1a), 48,000 (E3-BP) and 36,000 (E1β). Apparent Mr ~ 8,500,000 by sedimentation equilibrium centrifugation.

Supplied: 10 μg in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 10% glycerol. Maintain in aliquots at -70° C. Avoid repeated thawing.

Activity: ~ 12-18 μmol of NADH formed per min per mg of protein with pyruvate as substrate.

Synonyms: Pyruvate Dehydrogenase Complex

Background: The pyruvate dehydrogenase complex (PDC) is a mitochondrial multienzyme complex which catalyzes the oxidative decarboxylation of pyruvate. It is regulated by reversible phosphorylation.  Phosphorylation and inactivation of PDC is catalyzed by a kinase which is  tightly associated with the complex. Dephosphorylation and concomittant reactivation of the complex is catalyzed by loosely associated mitochondiral protein phosphatases.


Related Products:   PDC (50 μg) ♦ BCKDC (50 μg) ♦ BCKDC (100 μg) ♦ BCKD Kinase Kit

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