Jun (10 μg)

Source: Recombinant human produced in E. coli

Purity: > 90% by SDS-PAGE, apparent Mr ~ 39-kDa

Supplied: 10 μg in 50 μl of 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 10% glycerol. Maintain in aliquots at -70° C. Avoid repeated thawing.

Background: c-Jun is an important proto-oncogene and transactivating factor that is a component of the AP-1 and ATF-2 transcription factors. c-Jun is acutely regulated by a wide variety of cellular signals via modulation of its phosphorylation state, which can both inhibit and activate the proto-oncogene. Phosphorylation by GSK-3 at a region proxim al to the highly basic DNA binding domain (Thr239/Ser243/Ser249) prevents DNA association with c-Jun homodimers, but not with c-Jun-c-Fos heterodimers. Phosphorylation catalyzed by JNK/SAPK at Ser63 and Ser73, in a region proximal to the transactivation domain, activates the c-Jun/AP-1 and c-Jun/ATF2 transcriptional activator complexes to induce a number of genes. The MAP kinases ERK1 and ERK2 also phosphorylate c-Jun at Thr91/Thr93/Thr95.

GloboZymes c-Jun preparations are suitable for studies on the kinetics, enzymology and regulation of the phosphorylation of this important proto-oncogene. The purified preparations are effective as substrate for JNK/SAPK, MAPK, GSK-3 and CK-2 (GLO112-001) among other protein kinases. 


Related Productsc-Jun (1 μg) ♦  c-Jun (5 μg) ♦ c-Jun GST (1 μg) ♦ c-Jun GST (5 μg) ♦ c-Jun GST (10 μg) ♦ CK2  

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