$165.00

eIF4E(S53A) 5 μg
[GLO122-005]

Source: Recombinant human produced in E. coli

Purity: > 90% by SDS-PAGE, apparent Mr ~ 28-kDa

Supplied: 5 μg in 50 μl of 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 10% glycerol. Maintain in aliquots at -70° C. Avoid repeated thawing.

Synonym: Eukaryotic Protein Synthesis Initiation Factor 4E Serine 53 to Alanine Mutant; eIF-4ES53A.

Background: eIF4E is a mRNA Cap-binding protein involved in an early rate limiting step in the initiation of protein synthesis. eIF4E undergoes phosphorylation at Ser209 in response to growth factors and other extracellular stimuli. This reaction is thought to be catalyzed in cells by the mitogen-activated protein (MAP) kinase interacting kinases (Mnk's) and an insulin-stimulated cytosolic protamine kinase (cPK, GLO117-100) which is activated by members of the MAP kinase family. eIF4E also undergoes phosphorylation at Thr210 following incubation with cPK. Preparations of eIF4ES53A undergo phosphorylation at Ser209 and Thr210. However, because eIF4ES53A is thought to be inactive, preparations of this mutant are useful as control in studies to determine the function of the native protein.

Product Datasheet (pdf) 

References: J Biol Chem 270, 14824-14828 ♦  J Biol Chem 270, 14597-14603 ♦ Biochime 76, 822-830 ♦  J Biol Chem 271, 11831-11837 ♦  J Biol Chem 270, 21684-21688

Related Products: eIF4E (5 μg) ♦ eIF4E (15 μg) ♦ eIF4E (30 μg) ♦  eIF4E(S209A) (5 μg) ♦ eIF4E(S209A) (15 μg) ♦ eIF4E(S209A) (30 μg) ♦ eIF4E(T210A) (5 μg) ♦ eIF4E(T210A) (15 μg) ♦ eIF4E(T210A) (30 μg)eIF4E(S209T210A) (5 μg) ♦ eIF4E(S209T210A) (15 μg) ♦ eIF4E(S209T210A) (30 μg)eIF4E(S53A) (5 μg) ♦  eIF4E(S53A) (15 μg) ♦ eIF4E(S53A) (30 μg) ♦ eIF4E(S124A) (5 μg) ♦ eIF4E(S124A) (15 μg) ♦ eIF4E(S124A) (30 μg) ♦ cPK

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