Description: The PP2AC Detection Kit includes 200 μl of Anti-PP2AC (GLO148-200) and an aliquot of highly purified bovine kidney protein phosphatase 2A (see GLO130-GLO132). The enzyme aliquot is provided in SDS sample buffer ready for use in five applications as positive control in Western blots. Anti-PP2AC was raised in rabbits to the catalytic subunit of PP2A.
Synonyms: Protein Phosphatase 2A Catalytic Subunit Western Blot Detection Kit.
Background: Protein phosphatase 2A (PP2A, GLO130-GLO132) is a divalent cation-independent protein serine/ threonine phosphatase involved in regulating numerous cellular processes including the cell cycle, growth and differentiation. Physiological targets of PP2A include cell surface receptors and ion channels, protein kinases involved in mitogenic signaling and the cell cycle, key regulatory enzymes and proteins involved in metabolism, as well as numerous transcription factors. The phosphatase has been implicated as a growth suppressor. It is inhibited potently by tumor promoters such as okadaic acid and by I1PP2A (GLO140-001) and I2PP2A (SET) (GLO141-001), two naturally-occurring cancer-associated cellular proteins. Additionally, the SV40 small t antigen replaces the B subunit during viral transformation and subverts the physiological function of a subset of PP2A complexes in a substrate selective manner.
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References: Biochem J 287, 1019-1022 ♦ Proc Natl Acad Sci USA 90, 2500-2504 ♦ Trends Cell Biol 4, 287-291 ♦ Ann Rev Cell Biol 10, 55-86 ♦ Biochem J 353, 417
Related Products: Anti-PP2AC (100 μl) ♦ Anti-PP2AC (200 μl) ♦ PP2A1 (1 μg) ♦ PP2A1 (5 μg) ♦ PP2A2 (1 μg) ♦ PP2A2 (5 μg) ♦ PP2AC (1 μg)