$277.20

Anti-PAK (100 μg)
[GLO152-050]

Description: Anti-PAK is an affinity-purified polyclonal antibody produced in rabbits to a synthetic peptide corresponding to residues 484-502 of human PAK1. The antibody is useful for Western blotting, for example, with 30 μg HEK293 cell extracts at 1:1000 dilution of the antibody. In addition to PAK1, the antibody displays some reactivity with PAK2.

Synonyms: Anti-p65PAK; Anti-p68PAK; Antibody to p21-Activated Protein Kinase.

Background: The p21-GTPase-activated protein kinases (PAK’s) represent a family of enzymes that are involved in regulating cell morphology and cytoskeleton organization, cell motility, apoptosis, neuronal functions, cell cycle progression, viral replication and numerous other signal transduction processes. PAK’s have been found to interact with protein phosphatase 2A (PP2A, GLO130-GLO132), ribosomal protein S6 kinase, Mixed Lineage Kinase (MLK2), the SH3-SH2 adapter Nck and other regulatory proteins. Physiological targets of PAK include p38 MAP kinase, JNK, Raf1 and Synapsin I. The catalytic domains of PAK family members are produced in apoptotic cells via a caspase-dependent pathway. Unlike the native enzymes, the catalytic domains do not require Rac or Cdc42 (GLO128-005, GLO128-025, GLO128-050) to undergo autophosphorylation and activation.

Product Datasheet (pdf) 

References:

  1. Nature 367: 40
  2. J Biol Chem 270: 21695

Related Products:  Anti-PAK (200 μg) ♦  Anti-AK/PAK (100 μg) ♦  Anti-AK/PAK (200 μg) ♦ PAK Detection Kit ♦ PAK1 (1 μg) ♦ PAK1 (5 μg) ♦ PAK1 (10 μg) ♦Cdc42 (5 μg) ♦ Cdc42 (25 μg) ♦ Cdc42 (50 μg)MBP (10 mg)  ♦ MBP (25 mg)   

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