$625.00

AK/PAK2 Detection Kit
[GLO151-01]

Description: The AK/PAK2 Detection Kit includes 200 μg of affinity-purified Anti-AK/PAK2 and a sample of highly purified AK/PAK2. The AK/PAK2 sample is provided in SDS sample buffer ready for use in five applications as positive control in Western blots. The polyclonal antibody was produced in rabbits to a synthetic peptide corresponding to residues 273-291 of the catalytic domain of human lymphocyte PAK2.

Synonyms: p21-Activated Protein Kinase PAK2 Catalytic Domain Western Blot Detection Kit.

Background: The catalytic domain of PAK2 was originally isolated as a relatively rare auto-activated protein kinase (AK), so termed because the purified enzyme underwent rapid autophosphorylation and concomitant 15-30-fold activation. Subsequent discovery of the PAK family of enzymes and amino sequencing of AK revealed that AK was equivalent to the catalytic domain of PAK2. Unlike the reported activity of native PAK2, AK does not bind nor is it stimulated by the p21-GTPases Cdc42 (GLO128-005GLO128-025GLO128-050) and Rac. AK is a physiologically relevant form of PAK2. It is produced from the native enzyme via a caspase-dependent pathway in cells undergoing apoptosis.

References:

  1. Proc Natl Acad Sci USA 90: 2500
  2. J Biol Chem 268: 11193
  3. J Cell Physiol 178: 397
  4. J Biol Chem 276: 14909

Related ProductsAnti-AK/PAK2 (100 μg) ♦ Anti-AK/PAK2 (200 μg) ♦  AK/PAK2 (1 Unit) ♦  AK/PAK2 (2 U)  ♦ AK/PAK2 Assay Kit ♦ PAK1 (1 μg) ♦ PAK1 (5 μg) ♦ PAK1 (10 μg) ♦ Cdc42 (5 μg) ♦ Cdc42 (25 μg) ♦ Cdc42 (50 μg) ♦ MBP (10 mg)  ♦ MBP (25 mg) 

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